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RealPCR BTV/EHDV RNA Mix

Bluetongue virus (BTV) and epizootic hemorrhagic disease virus (EHDV)

The IDEXX RealPCR BTV/EHDV RNA Mix is used for the identification of bluetongue virus (BTV) RNA and epizootic hemorrhagic disease virus (EHDV) RNA when combined with the shared reagents of the IDEXX RealPCR platform. Samples can be tested simultaneously with any other IDEXX RealPCR reagents using shared reagents and a single real-time PCR program.
An internal control is part of that mix and is based on the detection of endogenous ruminant (bovine, ovine and caprine) RNA present in the sample and is referred to as the internal sample control (ISC).

* Availability / Distribution: Worldwide

Test details

Features

Identification of BTV RNA and EHDV RNA in a single reaction
Results in approximately 70 minutes

Part numbers and sizes

99-56695 (100 reactions)

About bluetongue and epizootic hemorrhagic disease

Bluetongue is a viral disease affecting bovines, ovines, caprines and wild ruminants. The disease is transmitted by an insect from the genus Culicoïdes and clinical signs can be associated with intense inflammation, high fever, tongue and face congestion and edemas, ulcerations and mucosal hemorrhages. The disease is caused by the bluetongue virus (BTV), a segmented RNA orbivirus from the reoviridae family. Multiple serotypes have been identified.

Epizootic hemorrhagic disease is a viral disease caused by epizootic hemorrhagic disease virus, (EHDV) which is closely related to BTV. Similar to BTV, it affects mainly cattle and wild ruminants, although small ruminants can also carry the virus. Transmission is by the same vector as BTV, and clinical signs include fever, anorexia, lameness, respiratory distress and potential death. There are multiple identified serotypes of EHDV.

Early detection and differentiation of BTV and EHDV using real-time PCR can limit the spread of the disease and the associated economic losses, particularly by testing animals before moving them between regions or countries.