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Quanti-Tray System

Take the guesswork out of bacterial counts


                   

  • Provides easy, rapid, and accurate bacterial counts.
  • Quanti-Tray and Quanti-Tray/2000 are used when quantifying coliforms, E. coli, enterococci, Pseudomonas aeruginosa, and Heterotrophic Plate Counts (HPC).
  • The Quanti-Tray/Legiolert is only used to quantify Legionella pneumophila.
  • The Quanti-Tray System delivers semiautomated quantification methods based on the Most Probable Number (MPN) model.
  • Learn about the Quanti-Tray Sealer PLUS and our generous trade-in program.

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Overview

Easy
  • No media preparation.
  • No pipetting.
  • No dilutions (for counts up to 200 with Quanti-Tray; 2,419 with Quanti-Tray/2000; and up to 2,272 with Quanti-Tray/Legiolert).

Rapid
  • Less than 1 minute of hands-on time.
  • Results in 24 hours or less (7 days for Legiolert).
  • No confirmation necessary.
Accurate
  • Detects down to one organism per 100 mL.
  • 95% confidence limits better than 5- or 10-tube Most Probable Number (MPN).
  • 95% confidence limits better than or comparable to membrane filtration (MF).
     
Cost effective
  • Minimal equipment to purchase and maintain.
  • No glassware to purchase and clean.
  • Minimal bench space needed.

Science

How Quanti-Tray works

Quanti-Tray

With Quanti-Tray, a 100 mL sample is divided into 51 wells. The Most Probable Number (MPN) approach is used to determine the number of bacteria in the original sample. Automation minimises hands-on time and maximises throughput (up to 4 samples per minute). The relatively large number of wells provides a high counting range of 1–200 (without dilutions) and very tight 95% confidence limits.

Quanti-Tray/2000

The Quanti-Tray/2000 is based on the same statistical model as the traditional 15-tube serial dilution. With the Quanti-Tray/2000, the sample is automatically divided into the proper portions when sealed by the Quanti-Tray Sealer PLUS. The Quanti-Tray system does not require the use of test tubes, Durham tubes, or any dilutions. By automatically distributing the sample into 97 wells of 2 different sizes, Quanti-Tray/2000 yields a counting range of 1–2,419 with a far better 95% confidence limit than a 15-tube serial dilution.

Quanti-Tray/Legiolert

With Quanti-Tray/Legiolert, a 100 mL sample is divided into 96 wells. The Standard Methods’ Most Probable Number (MPN) approach is used to determine the number of bacteria in the original sample, using the MPN tables provided. Automation minimises hands-on time and maximises throughput (up to four samples per minute). The 96-well tray provides a counting range of 1–2,272 (without dilutions).

 

Quanti-Tray Sealer PLUS

The Quanti-Tray Sealer PLUS is the next generation sealer in the Quanti-Tray system. The sealer makes quantification with Quanti-Tray and Quanti-Tray/2000 possible while saving time and improving laboratory efficiency. Features include a 2–3 minute warm-up time, faster sealing of trays, energy efficiency, and an access panel that makes cleaning easy.

 

How to use

Instructions


Step 1

Add sample/reagent to tray.

Step 2

Seal tray in Quanti-Tray Sealer PLUS.

Step 3

Incubate, count positive wells, and refer to appropriate MPN table.

Frequently asked questions

 

Quanti-Tray provides a wider counting range than and comparable accuracy to membrane filtration (MF).

Method Lower Counting Range/100mL. Upper Counting Range/100 mL.
Quanti-Tray <1 200
Quanti-Tray/2000 <1 2,419
Quanti-Tray/Legiolert <1 2,272
5-Tube MPN <1.1 16
10-Tube MPN <1.1 23
Membrane Filtration <1 80
     

Quanti-Tray and Quanti-Tray/2000 are gas-sterilized using ethylene oxide. Quanti-Tray/Legiolert is treated using electron-beam technology to be Legionella pneumophila free.

The shelf life of Quanti-Tray and Quanti-Tray/2000 is up to 3 years from date of manufacture. The shelf life of Quanti-Tray/Legiolert is up to 1 year from date of manufacture. When storing opened bags of trays, fold over the opened end of the bag and seal with tape.

No, the short time period that the tray is in the sealer will not harm the bacteria. The temperature in the wells will not increase more than 5°C during sealing.

One or two empty wells do not affect the test interpretation as long as the entire sample is in the tray. The effect on the Most Probable Number (MPN) is statistically insignificant. A partially filled well is interpreted the same way as a full well.

Yes, Quanti-Trays can be stacked up to 10 trays high. Use caution not to block the airflow in the incubator. Quanti-Tray/Legiolert trays must be stacked paper side down (with wells facing up) and should be oriented in alternating directions to increase stability.

Please refer to the package insert to determine result interpretation.

Colour intensity is a reflection of the different metabolic rates and final concentrations of the coliforms and E. coli in the different wells. Any yellow colour or fluorescence in the sample well equal to or greater than the Quanti-Tray or Quanti-Tray/2000 Comparator is considered positive.

To determine the presence of E. coli, wells must be yellow and fluoresce. A fluorescent well that is not yellow is not an E. coli-positive well.

No, Quanti-Tray/Legiolert can only be used with Quanti-Tray Sealer PLUS.

Resources & Tools

Tests & Accessories

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Product Information

Quanti-Tray 51-Well Trays (100 pack)
Product Number: 98-21378-00
Catalogue Number: WQT-100

Quanti-Tray/2000 97-Well Trays (100 pack)
Product Number: 98-21675-00
Catalogue Number: WQT-2K

Quanti-Tray/Legiolert (20 pack)
Product Number: 98-0005796-00
Catalogue Number: WQTLGT-20

Quanti-Tray/Legiolert (100 pack)
Product Number: 98-0005754-00
Catalogue Number: WQTLGT-100

Resources

IDEXX Water has reference materials and approval documents to support the many products in our water portfolio. Find the document(s) you need by selecting the link below.

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Water Customer Support

Australia & New Zealand Head Office

IDEXX Laboratories Pty Ltd.
Unit 6, 38-46
South Street
Rydalmere, NSW 2116
AUSTRALIA

Phone (AUS): 1300 44 33 99
Phone (NZ): 0800 838 522

Fax (AU): 1800 634 409
Fax: (NZ): 0800 448 443

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