Pseudalert®

24-hour detection of Pseudomonas aeruginosa

 
Bottled Water Pool-Spa Water Drinking Water
 

Detects or quantifies Pseudomonas aeruginosa in a 100 ml format. Results in 24 hours.

 

Use the Pseudalert® Test to detect P. aeruginosa quickly to safeguard against "hot-tub rash (folliculitis)," "swimmer’s ear" and a host of other infections, avoiding illness in sensitive populations.

  • Detects P. aeruginosa in 24 hours.
  • Delivers definitive results with no additional confirmation steps.
  • Minimises the risk of false positives from nontarget organisms.
  • Same easy-to-use platform as Colilert®.
 
Pseudalert
 

Questions?

Call 1300 4 IDEXX or email water@idexx.com.

Pseudalert: Be confident in your outcomes

 

 

Easy

  • Blue fluorescence of wells or vessel indicates a positive result.
  • Same familiar platform as the Colilert® test:
    • Ready-to-use reagents, no media preparation.
    • Convenient unit-dosed packaging.
    • No toxic compounds.
  • Use with Quanti-Tray® or Quanti-Tray®/2000 for most probable number (MPN) enumeration.
 

Rapid

  • Definitive results in 24 hours.
  • Less than 1 minute of hands-on time for streamlined testing work flow.
 

Accurate

  • Specifically detects Pseudomonas aeruginosa, reducing the risk of false positives from nontarget organisms.
  • Results are definitive without additional confirmation steps.
  • Simple blue fluorescent change minimises subjective result interpretation.
Resource List
 
 
Product Information

Pseudalert® (20-test pack)
Catalog Number: WPSE020I

Pseudalert® (200-test pack)
Catalog Number: WPSE200I

Sample size: 100 ml

Time to results: 24 hours

Shelf life: Up to 12 months at 2–30°C; store away from direct light

Read: Sample fluoresces to indicate the presence of Pseudomonas aeruginosa

Uses: Presence/absence or quantification testing using IDEXX Quanti-Tray® or Quanti-Tray®/2000

 

Snap packs for 100-ml water samples:

  • 20 test pack No.WPSE0201
  • 100 test pack No.WPSE100I
  • 200 test pack No.WPSE2001

How Pseudalert® works

 

The Pseudalert test detects the presence of Pseudomonas aeruginosa in water samples.

 

The test is based on a bacterial enzyme detection technology that signals the presence of P. aeruginosa through the hydrolysis of a substrate in the Pseudalert reagent. P. aeruginosa cells rapidly grow and reproduce using the rich supply of amino acids, vitamins and other nutrients present in the Pseudalert reagent. Actively growing strains of P. aeruginosa have an enzyme that cleaves the substrate in the reagent to produce blue fluorescence under ultraviolet light.

 

Pseudalert detects P. aeruginosa at 1 cfu per 100 ml water sample within 24 hours.

 

 

View/Print Pseudalert Procedure 

 
 
Learn how to use Pseudalert and Pseudalert 250
 

A step-by-step guide to using Pseudalert

 
Presence/Absence (P/A)
 
Quantification 
       
 

Step 1

Add reagent to sample (sample will turn yellow), and then incubate at 38±0.5 °C for 24-28 hours.

  

Step 1

Add reagent to sample (sample will turn yellow).

 
       
 

Step 2

Read under ultraviolet (UV) light. Blue fluorescence indicates presence of  P. aeruginosa. See the Pseudalert Quick Reference Guide (PDF) for tips on reading fluorescence.

  

Step 2

Add IDEXX Antifoam Solution to vessel.

 
       
     

Step 3

Pour into Quanti-Tray® (counts from 1–200) or Quanti-Tray®/2000 (counts from 1–2,419).

 
       
     

Step 4

Seal using a Quanti-Tray® Sealer, and then incubate at 38±0.5 °C for 24–28 hours.

 
       
     

Step 5

Read under ultraviolet (UV) light. Blue fluorescence indicates the presence of  P. aeruginosa. Count the positive (blue fluorescent) wells and refer to MPN table. See the Pseudalert Quick Reference Guide (PDF) for tips on reading fluorescence.

 
 

Frequently asked questions and answers

 

What water types can be tested with the Pseudalert test?

The Pseudalert test is designed for water samples including bottled, pool and spa water. Bottled water samples are defined as samples taken from any point in the manufacturing of bottled water (from the original water to the finished product). Extensive studies were done on Pseudomonas aeruginosa stressed by the various chemicals and cleaning agents used in pools and spas to mimic the impact of treatment. Pseudalert was specifically formulated and tested to ensure it would detect stressed P. aeruginosa in these samples.

 

How does Pseudalert work?

Pseudalert is based on a bacterial enzyme detection method that signals the presence of Pseudomonas aeruginosa through the hydrolysis of an enzyme substrate. P. aeruginosa cells rapidly grow and reproduce using the rich supply of amino acids, vitamins and other nutrients present in the Pseudalert reagent. Actively growing strains of P. aeruginosa have an enzyme that cleaves the substrate in the reagent to produce blue fluorescence under ultraviolet light. Pseudalert detects P. aeruginosa at 1 cfu per 100 mL or 250 mL sample within 24 hours.

 

Why does Pseudalert detect only Pseudomonas aeruginosa and not other Pseudomonas species?

Pseudomonas aeruginosa is the only Pseudomonas species known to cause health issues. For this reason, regulations in many countries apply specifically to P. aeruginosa detection.

 

What is the detection limit of Pseudalert?

Pseudalert detects Pseudomonas aeruginosa at 1 cfu/100 mL or 1 cfu/250 mL.

 

Is there a comparator for Pseudalert?

There is no comparator for Pseudalert. For comparison, use a negative control when interpreting results.

 

What is the incubation time and temperature for samples tested with Pseudalert?

Water samples are incubated at 38°C +/– 0.5°C for 24 hours, and results are valid up to 28 hours.

 

Can I incubate at a temperature other than the recommended 38°C?

No. Breakthrough of nontarget organisms can occur at temperatures other than 38°C ±0.5°C.

 

Are results valid prior to 24 hours?

Yes, if the results are positive. An inoculated Pseudalert presence/absence sample that is positive for Pseudomonas aeruginosa before 24 hours is a confirmed positive test for P. aeruginosa. However, for an accurate quantitative determination of P. aeruginosa in a sample, the results should be read at 24–28 hours of incubation.

 

Are results valid after 28 hours?

Yes, if the results are negative. If an inoculated Pseudalert sample is inadvertently incubated for over 28 hours, lack of blue fluorescence is a valid negative test result. However, blue fluorescence that develops after 28 hours is not a valid positive test result; the test should be repeated or verified.

 

Can either a 51-well Quanti-Tray or a 97-well Quanti-Tray/2000 be used with Pseudalert?

Yes, both the Quanti-Tray and Quanti-Tray/2000 can be used with Pseudalert in the 100 mL format but not the 250-mL format.

 

Which vessels should I use with Pseudalert?

For presence/absence testing, use a sterile, transparent, nonfluorescing vessel. For the Quanti-Tray and Quanti-Tray/2000 systems, any sterile vessel can be used.

 

What are the recommended storage conditions for Pseudalert?

Store Pseudalert at 2–30°C away from light.

 

What color and consistency should the Pseudalert powder be?

The Pseudalert reagent powder should be tan in color and free-flowing. If you have any concerns about the color or integrity of the powder, contact IDEXX Technical Services.

 

Why is antifoam solution needed when testing samples with Pseudalert in the Quanti-Tray or Quanti-Tray/2000?

The mixing of sample and reagent can create foaming. IDEXX Antifoam Solution is needed to help the foaming subside before sealing the sample in the Quanti-Tray® or Quanti-Tray/2000®.

 

Pseudalert: Everything you need for confident outcomes

 
 

Resource Library for Pseudalert

 

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