24-hour detection of Pseudomonas aeruginosa
Detects or quantifies Pseudomonas aeruginosa in a 100 ml format. Results in 24 hours.
Use the Pseudalert® Test to detect P. aeruginosa quickly to safeguard against "hot-tub rash (folliculitis)," "swimmer’s ear" and a host of other infections, avoiding illness in sensitive populations.
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Pseudalert: Be confident in your outcomes
Pseudalert® (20-test pack)
Pseudalert® (200-test pack)
Sample size: 100 ml
Time to results: 24 hours
Shelf life: Up to 12 months at 2–30°C; store away from direct light
Read: Sample fluoresces to indicate the presence of Pseudomonas aeruginosa
Snap packs for 100-ml water samples:
How Pseudalert® works
The Pseudalert test detects the presence of Pseudomonas aeruginosa in water samples.
The test is based on a bacterial enzyme detection technology that signals the presence of P. aeruginosa through the hydrolysis of a substrate in the Pseudalert reagent. P. aeruginosa cells rapidly grow and reproduce using the rich supply of amino acids, vitamins and other nutrients present in the Pseudalert reagent. Actively growing strains of P. aeruginosa have an enzyme that cleaves the substrate in the reagent to produce blue fluorescence under ultraviolet light.
Pseudalert detects P. aeruginosa at 1 cfu per 100 ml water sample within 24 hours.
View/Print Pseudalert Procedure
A step-by-step guide to using Pseudalert
Add reagent to sample (sample will turn yellow), and then incubate at 38±0.5 °C for 24-28 hours.
Add reagent to sample (sample will turn yellow).
Read under ultraviolet (UV) light. Blue fluorescence indicates presence of P. aeruginosa. See the Pseudalert Quick Reference Guide (PDF) for tips on reading fluorescence.
Add IDEXX Antifoam Solution to vessel.
Seal using a Quanti-Tray® Sealer, and then incubate at 38±0.5 °C for 24–28 hours.
Read under ultraviolet (UV) light. Blue fluorescence indicates the presence of P. aeruginosa. Count the positive (blue fluorescent) wells and refer to MPN table. See the Pseudalert Quick Reference Guide (PDF) for tips on reading fluorescence.
Frequently asked questions and answers
The Pseudalert test is designed for water samples including bottled, pool and spa water. Bottled water samples are defined as samples taken from any point in the manufacturing of bottled water (from the original water to the finished product). Extensive studies were done on Pseudomonas aeruginosa stressed by the various chemicals and cleaning agents used in pools and spas to mimic the impact of treatment. Pseudalert was specifically formulated and tested to ensure it would detect stressed P. aeruginosa in these samples.
Pseudomonas aeruginosa is the only Pseudomonas species known to cause health issues. For this reason, regulations in many countries apply specifically to P. aeruginosa detection.
Pseudalert detects Pseudomonas aeruginosa at 1 cfu/100 mL or 1 cfu/250 mL.
There is no comparator for Pseudalert. For comparison, use a negative control when interpreting results.
Water samples are incubated at 38°C +/– 0.5°C for 24 hours, and results are valid up to 28 hours.
No. Breakthrough of nontarget organisms can occur at temperatures other than 38°C ±0.5°C.
Yes, if the results are positive. An inoculated Pseudalert presence/absence sample that is positive for Pseudomonas aeruginosa before 24 hours is a confirmed positive test for P. aeruginosa. However, for an accurate quantitative determination of P. aeruginosa in a sample, the results should be read at 24–28 hours of incubation.
Yes, if the results are negative. If an inoculated Pseudalert sample is inadvertently incubated for over 28 hours, lack of blue fluorescence is a valid negative test result. However, blue fluorescence that develops after 28 hours is not a valid positive test result; the test should be repeated or verified.
For presence/absence testing, use a sterile, transparent, nonfluorescing vessel. For the Quanti-Tray and Quanti-Tray/2000 systems, any sterile vessel can be used.
Store Pseudalert at 2–30°C away from light.
The Pseudalert reagent powder should be tan in color and free-flowing. If you have any concerns about the color or integrity of the powder, contact IDEXX Technical Services.