HPC for Quanti-Tray®
Heterotrophic plate count made easy for bottling and manufacturing plants
Simpler detection and quantification of heterotrophic organisms
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HPC for Quanti-Tray: Confidence in quantification
Sample size: 100 ml
Time to results: 44–72 hours
Shelf life: Up to 12 months at 2–25°C; store away from direct light
Read: Sample fluoresces to indicate the presence of heterotrophs
Snap packs with Quanti-Tray for 100-ml water samples:
Snap packs with Quanti-Tray/2000 for 100-ml water samples:
How HPC for Quanti-Tray® works
The HPC for Quanti-Tray® Test is used for the quantification of heterotrophic organisms in 100 ml of water.
The test is based on IDEXX-patented Multiple Enzyme Technology™, which detects viable heterotrophic organisms by testing for the presence of key enzymes known to be present in these organisms. The test uses multiple enzyme substrates that produce blue fluorescence when metabolised by water-borne bacteria.
The sample/reagent mixture is added to a Quanti-Tray®, incubated and then examined for fluorescing wells. The number of fluorescing wells corresponds to a most probable number (MPN) of total heterotrophic organisms in the original sample.
The HPC for Quanti-Tray Test detects organisms at 1 cfu/100 ml after 44–72 hours of incubation.
A step-by-step guide to using HPC for Quanti-Tray
Frequently asked questions and answers
The HPC for Quanti-Tray® Test is designed specifically for bottled-water samples. Bottled-water samples are defined as samples taken from any point in the bottled-water manufacturing process (from the original water source, to transportation vehicles, to the manufacturing line, to the finished product).
Water samples are incubated at 36ºC (±2ºC) for 44 hours, and results are valid up to 72 hours.
The HPC for Quanti-Tray Test detects heterotrophic organisms at 1 cfu/100 mL.
There is no comparator for the HPC for Quanti-Tray Test. Use a negative control for comparison when you interpret results.
Yes, if the results are positive. An inoculated HPC for Quanti-Tray Test sample that is positive before 44 hours is a confirmed positive test. However, for an accurate quantitative determination of heterotrophic count in a sample, the results should be read between 44 and 72 hours of incubation.
Yes, if the results are negative. If an inoculated HPC for Quanti-Tray Test sample is inadvertently incubated over 72 hours, lack of fluorescence is a valid negative test. However, a fluorescent read after the 72 hours is not valid and should be repeated or verified.
Yes, both the Quanti-Tray® and Quanti-Tray®/2000 can be used with the HPC for Quanti-Tray Test in the 100-mL format.
2–25ºC away from light.
The HPC for Quanti-Tray Test reagent powder should be pink in color and of a free-flowing, granular consistency. Direct any concerns about the color or integrity of the powder to IDEXX Technical Service.
Check with your local, state and/or federal authorities for proper disposal of bacteriological biohazard materials at your facility.
HPC for Quanti-Tray should not be used with carbonated water samples.
HPC for Quanti-Tray: Everything you need for confident outcomes
Note: Availability varies by country.