IDEXX RealPCR™ Tests

Definitive answers through accurate, fast real-time PCR

 

IDEXX’s Molecular Diagnostics Laboratory, directed by Christian Leutenegger, DrVetMed, PhD, FVH, offers real-time polymerase chain reaction (PCR) testing—providing you with convenient and affordable access to the accuracy of PCR as part of your routine test menu.

Polymerase chain reaction (PCR) is a simple yet elegant process that enables the production of virtually unlimited copies of genetic material in the laboratory. PCR testing identifies a pathogen based on the presence of the pathogen’s DNA or RNA in the patient sample.

IDEXX RealPCR tests are now run in our new Brisbane laboratory, with results usually available within 1-3 working days.

 

IDEXX is delighted to announce the opening of our veterinary specific RealPCRTM laboratory

RealPCR™ Tests
 
Complimentary internal medicine consultations. Contact us at 1300 44 33 99, option 1, option 2.

Exceptional accuracy is available and affordable

 

With IDEXX RealPCR™ tests, you can count on:

  • The latest innovation in PCR technology—Real-time PCR is a closed-tube system that delivers shorter assay run times and virtually eliminates false positives.
  • Fast answers—IDEXX makes results available in just 1 to 3 days, so you can work through your list of differential diagnoses faster.
  • Surprising cost savings—IDEXX RealPCR tests and panels cost about the same as our other laboratory tests, making them much more affordable than send-out PCR tests.

Real-time PCR answers for your toughest cases

 

Discover the value of having real-time PCR answers for your toughest cases—with results in just 1-3 working days—by ordering these RealPCR tests, which are available as single tests, or in panels and profiles.

 

Canine and Feline

 

Canine and Feline Diarrhoea Panels and Tests

Quickly identify infectious causes for an all-too-common problem.

Cats and dogs frequently present with diarrhoea, but identifying causative agents often requires numerous tests with varying reliability. The IDEXX Canine and Feline Diarrhoea RealPCR™ Panels allow you to screen for and accurately identify multiple infectious causes of diarrhoea from only a single faecal sample.

Knowing precisely which intestinal pathogen is affecting your patient gives you the information you need to hone treatment recommendations and improve efficacy. And because it’s RealPCR technology, you can find out more quickly and with greater accuracy exactly what’s contributing to your patients’ diarrhoea.

 

When to use IDEXX Canine and Feline Diarrhoea RealPCR™ Panels:

  • To identify the pathogen(s) that may be causing or contributing to diarrhoea.
  • For timely diagnosis and determining appropriate therapies/treatments.
  • As a surveillance tool for dog or cat populations in shelters, breeding facilities, etc.*
  • To identify and minimise human exposure to zoonotic pathogens.

 

*
IDEXX diarrhoea RealPCR panels are not recommended as a screen for healthy pets except for in a shelter- or boarding-type environment.

 

Canine and Feline Diarrhoea Panels

Test CodeName and Contents

CDSP

Canine Diarrhoea RealPCR™ Panel

Canine distemper virus, canine enteric coronavirus (CECoV), canine parvovirus 2 (CPV-2), Clostridium perfringens enterotoxin A gene, Cryptosporidium spp., Giardia spp. and Salmonella spp. RealPCR™ tests. If the RealPCR™ test is positive for Salmonella spp., a culture and susceptibilities will be automatically performed at no additional charge.
Note: The reflex Salmonella spp. culture will only be performed if a sample in faecal culture transport media is received.

FDCP

Feline Diarrhoea RealPCR™ Panel

Clostridium perfringens enterotoxin A gene, Cryptosporidium spp., feline coronavirus (FCoV), feline panleukopenia virus, Giardia spp., Salmonella spp., Toxoplasma gondii and Tritrichomonas foetus RealPCR™ tests. If the RealPCR™ test is positive for Salmonella spp., a culture and susceptibilities will be automatically performed at no additional charge.
Note: The reflex Salmonella spp. culture will only be performed if a sample in faecal culture transport media is received.

 

Specimen requirements:
5 g fresh faeces (1 g minimum) in a sterile container; keep refrigerated; collect sample prior to antibiotic administration.

When ordering a panel that includes the Salmonella spp. RealPCR™ Test, also submit 3–5 g fresh faeces in faecal culture transport media for reflex Salmonella spp. culture if indicated.

 

Canine and Feline Respiratory Disease Panels, Tests and Profiles

Test for and identify the causative agents of canine respiratory disease and feline URD with real-time PCR—now includes new H1N1 Influenza Virus RealPCR™ Test

Canine Respiratory Disease

Multiple infectious agents can be a cause of respiratory disease in dogs. Prompt identification and treatment of these agents allows you to better manage these cases, may lead to less severe clinical signs and allows for appropriate isolation of infected dogs.

The IDEXX Canine Respiratory Disease (CRD) RealPCR™ Panel provides rapid, sensitive and specific identification for eight infectious agents—all at once.

From 4,700 samples submitted and tested with the CRD RealPCR Panel during a 12-month period, results were:1

  • Positive for any one of the eight infectious agents 53.1% of the time
  • Positive for coinfections with two or more infectious agents 43.5% of the time

 

Prevalence of each component in the CRD RealPCR Panel

Infectious agentPrevalence

Bordetella bronchiseptica

25.0%

Canine respiratory coronavirus (CRCoV)

15.9%

Canine adenovirus type 2

15.9%

Canine distemper virus (CDV)

15.7%

Canine parainfluenza virus

12.2%

Canine herpesvirus type 1 (CHV-1)

4.1%

Canine influenza virus (H3N8)

1.4%

H1N1 influenza virus

1 case detected

 

Feline Upper Respiratory Disease

Cats presenting with signs of feline upper respiratory disease (URD)—sneezing, oculonasal discharge, conjunctivitis with or without oral ulceration—are probably common in your practice. But until now, there have been no quick, accurate and affordable tests that could determine the causative agents.

Running the quick and easy IDEXX Feline Upper Respiratory Disease (URD) RealPCR™ Panel dramatically increases your chance of accurately identifying the origins of feline URD.

From 7,500 samples submitted and tested with the Feline URD RealPCR Panel during a 12-month period, results were:1

  • Positive for any one of the six infectious agents 71.0% of the time
  • Positive for coinfections with two or more infectious agents 42.4% of the time

 

Prevalence of each component in the Feline URD RealPCR Panel

Infectious agentPrevalence

Mycoplasma felis

42.9%

Feline herpesvirus type 1 (FHV-1)

26.5%

Feline calicivirus

26.0%

Bordetella bronchiseptica

10.7%

Chlamydophila felis

8.0%

H1N1 influenza virus

2 cases detected

 

The results from running this single-panel, real-time PCR test enable you to quickly take the next steps:

  • Choose the appropriate treatment
  • Quarantine animals as needed
  • Improve patient outcome

 

Reference
1.
Data on file at IDEXX Laboratories, Inc. Westbrook, Maine USA.

 

Canine and Feline Respiratory Panels, Tests and Profiles

Test CodeName and Contents

CRS

Canine Respiratory Disease (CRD) RealPCR™ Panel

Bordetella bronchiseptica, canine adenovirus type 2, canine distemper virus, canine herpesvirus type 1 (CHV-1), canine influenza virus (H3N8), canine parainfluenza virus, canine respiratory coronavirus (CRCoV), H1N1 influenza virus, Mycoplasma cynos and Streptococcus equi subsp. zooepidemicus RealPCR™ tests.

FUR

Feline Upper Respiratory Disease (URD) RealPCR™ Panel

Bordetella bronchiseptica, Chlamydophila felis, feline calicivirus, feline herpesvirus type 1 (FHV-1), H1N1 influenza virus and Mycoplasma felis RealPCR™ tests

 

Specimen requirements:

Deep pharyngeal swab (with visible organic material on swab; please rub firmly) and a conjunctival swab (wipe eye clean; swab inside of eyelid) in the same tube; collect sample prior to antibiotic administration. Please submit dry, plastic-stemmed swabs, without transport media, in a RTT or an empty, sterile tube; keep refrigerated. When acquiring a pharyngeal swab, precautions should be taken to prevent patient from biting and/or ingesting part of swab.

 

For feline patients:

  • When ordering a feline URD profile that includes a CBC, chemistry or T4, also submit 2 mL serum, 1 mL EDTA whole blood (LTT) and two blood smears.
  • When ordering a feline URD profile that includes a urinalysis, also submit 5 mL urine in a sterile container.

 

 

Canine

 

Canine Distemper Virus (CDV) Quant RealPCR™ Test

Accurately diagnose canine distemper virus during early infection in dogs who have been recently vaccinated.

Many dogs develop clinical signs of distemper within days of adoption from a kennel or shelter, where they have often received a recent vaccination. Because most distemper vaccines consist of attenuated strains, highly sensitive real-time PCR test systems will pick up the replicating vaccine strain in about 20% of dogs over the course of a few weeks after each immunization.1 Until now, interpreting a positive test result in recently vaccinated dogs was challenging.

 

The CDV Quant RealPCR™ Test provides a quantitative measure of the CDV viral load. The amount of virus during infection is typically exponentially more than would be detected because of recent vaccination. Therefore, this quantitative information allows the test to discriminate vaccine interference from infection with a wild-type strain of distemper virus.

 

Canine Distemper Virus (CDV) Test

Test CodeName and Contents

Coming Soon

Distemper Virus (CDV) Quant RealPCR™ Test—Canine
Includes quantification of distemper viral particles if indicated.

 

Note:

Use for dogs with respiratory signs consistent with canine distemper virus that have been vaccinated with a modified live vaccine within the previous 4 weeks. For dogs with clinical manifestations requiring other nonrespiratory types or in dogs for which vaccine interference is not a concern, please see Distemper Virus (CDV) RealPCR™ Test—Canine.

 

Specimen requirements:

Deep pharyngeal swab (with visible organic material on swab; please rub firmly) and a conjunctival swab (wipe eye clean, swab inside of eyelid) in the same tube. Please submit dry plastic-stemmed swabs, without transport media, in RTT or empty, sterile tube (keep refrigerated).

 

1.
Leutenegger CM, Crawford C, Levy J, Estrada M. Canine distemper virus quantification by real-time PCR allows to differentiate vaccine virus interference and wildtype infection. In: Proceedings from the 2011 ACVIM Forum; June 15–18, 2011; Denver, CO.

 

Canine Leptospira spp. Test

Early detection with no vaccine cross-reactivity

The IDEXX Leptospira spp. RealPCR™ Test enables an early and accurate diagnosis of canine leptospirosis. A potentially life-threatening disease, leptospirosis most commonly manifests in dogs as severe acute kidney disease. In addition, urinary shedding of leptospires by infected animals poses a significant zoonotic risk to dog owners.

 

The IDEXX Leptospira spp. RealPCR Test offers advantages over serological testing:

  • Early detection provides the ability to diagnosis acute infection prior to antibody development.
  • Detects urinary shedding in sick and healthy animals to aid in diagnosis and minimize zoonotic risk.
  • No cross-reactivity and no interference of results by currently available bacterin-based vaccines, as determined by a recent study performed at Cornell University College of Veterinary Medicine (Richard E. Goldstein, DVM, DACVIM, DECVIM-CA, oral communication, June 2009).

 

Leptospirosis should be considered in every dog presenting with acute renal disease, especially as early diagnosis and treatment improves the prognosis of infected animals. Use the test code below when ordering the IDEXX Leptospira spp. RealPCR Test.

 

Canine Leptospira spp. Test

Test CodeName and Contents

LPT

Leptospira spp. RealPCR™ Test

 

Specimen requirements:
2 mL EDTA whole blood (purple topped tube) and 2 mL urine in a sterile container; keep refrigerated; collect sample prior to antibiotic administration.

 

Vector-borne Disease Panels and Tests

For sick animal testing—The combination of serological and PCR methodologies offer convenient, affordable comprehensive testing

IDEXX Reference Laboratories has introduced several new panels for vector-borne disease testing that combine both serology and real-time polymerase chain reaction (PCR). These next-generation IDEXX Vector-borne Disease Panels take advantage of the benefits of both methodologies, making it easy and cost-effective for veterinarians to make complete and accurate diagnosis.

Combining serology and PCR increases diagnostic sensitivity and reduces the chance for false negatives. The IDEXX RealPCR™ component of the vector-borne disease panels detects pathogenic nucleic acid (DNA), identifying infectious agents of clinical disease as well as coinfections, and the serology portion of the panel may reveal exposure to multiple pathogens and provides an indication of the duration of infection. For Lyme disease, the IDEXX C6 technology is the preferred diagnostic method of Borrelia burgdorferi infection.

 

When to use Vector-borne Disease Panels:

  • A sick patient with clinical signs and/or laboratory abnormalities consistent with a vector-borne disease

    Examples:

    • An animal presenting with clinical signs of lethargy, anorexia, lameness and fever
    • A sick patient with clinical laboratory findings that include anemia, thrombocytopenia, leukocyte abnormalities and/or hyperglobulinemia
  • Patients with suspected subclinical infections as based upon history, physical examination, serological results and clinical laboratory findings.

    Example: Clinically healthy animals with a history of tick exposure, physical examination findings of organomegaly or lymphadenomegaly, and laboratory abnormalities such as thrombocytopenia or anemia

  • Patients previously diagnosed with a vector-borne disease and are in need of monitoring therapy

    • The Lyme Quant C6® Test is run as part of several IDEXX Vector-borne Disease Panels and can indicate waning antibody levels
    • For other pathogens, a negative PCR result indicates a reduction in pathogen load

 

Note: Positive serologic results alone in a clinically healthy animal with normal laboratory results is NOT an indication for performing an IDEXX Vector-borne Disease Panel.

 

Vector-borne Disease Panels and Tests

Test CodeName and Contents

TIC

Canine Tick/Vector Comprehensive RealPCR™ Panel

Anaplasma spp., Babesia spp., Bartonella spp., Canine Haemotropic Mycoplasma RealPCR™ tests and Borrelia spp Lyme Quant C6® Test

 

Specimen requirements:
2 mL EDTA whole blood (LTT); keep refrigerated; collect sample prior to antibiotic administration.

 

When ordering a panel that includes a Lyme Quant C6 Test or FeLV antigen and FIV antibody by ELISA, also submit 1 mL serum.

 

 

Feline

 

Feline Coronavirus (FCoV) Test

Feline Coronavirus (FCoV) Test

Test CodeName and Contents

FCV

Feline Coronavirus (FCoV) RealPCR™ Test

 

Specimen requirements:
Ideal sample depends on clinical manifestation. (0.3 mL mimimum) pleural or abdominal effusion (preferred specimen), CSF, EDTA whole blood, or 10g faeces, fresh, frozen tissue (spleen, liver, kidney, lung), tissue embedded in parrafin, or tissue aspirates. Submit fluids in plain (red topped tube) or sterile container; keep refrigerated.

 

Feline Haemotropic Mycoplasma (FHM) Tests and Profiles

(formerly Haemobartonella spp.)
Eliminate the challenges in diagnosing feline anemia

Feline haemotropic mycoplasma (FHM) is a common cause of severe hemolytic anemia in cats.

 

In a recent nationwide study:1

  • 12.7% of healthy blood donor pet cats were infected with feline haemotropic mycoplasma species
  • A higher rate of FHM infection was found in outdoor cats and cats exposed to fleas

 

Until now, diagnosing feline haemotropic mycoplasmosis (formerly haemobartonellosis or feline infectious anemia) has been a challenge. The pathogenicity of FHM species differs:

  • Most pathogenic: Mycoplasma haemofelis (formerly Haemobartonella felis)
  • Moderately pathogenic: Candidatus Mycoplasma turicensis
  • Least pathogenic: Candidatus Mycoplasma haemominutum

 

Because of these differences in pathogenicity, the Feline Haemotropic Mycoplasma (FHM) RealPCR™ tests and profiles help you determine the significance of an FHM infection, which may affect case management, including the pursuit of additional diagnostics and treatment.

 

Visual slide review cannot distinguish FHM species, but real-time PCR can. Compare these two methods below to see how real-time PCR detects infections that a manual slide review can miss:2

IDEXX RealPCR vs. Slide Review by Trained Technicians

(Total samples = 303a)

 Positives found by IDEXX RealPCRPositives found by slide review
Positive for any species

88 (29.0%)

10 (3.3%)

Positives by species or species combination

Mhf: 21 (6.9%)

CaMh: 41 (13.5%)

CaMt: 5 (1.7%)

Mhf & CaMh: 8 (2.6%)

Mhf & CaMt: 3 (1.0%)

CaMh & CaMt: 3 (1.0%)

Mhf & CaMh & CaMt: 7 (2.3%)

Could not distinguish species

a.
All samples were whole blood from cats with hematocrit less than 25%.
Mhf = Mycoplasma haemofelis
CaMh = Candidatus Mycoplasma haemominutum
CaMt = Candidatus Mycoplasma turicensis

 

References
1.
Hackett TB, Jensen WA, Lehman TL, Hohenhaus AE, Crawford PC, Giger U, Lappin MR. Prevalence of DNA of Mycoplasma haemofelis, ‘Candidatus Mycoplasma haemominutum,’ Anaplasma phagocytophilum, and species of Bartonella, Neorickettsia, and Ehrlichia in cats used as blood donors in the United States. JAVMA. 2006;229(5):700–705.
2.
Data on file at IDEXX Laboratories, Inc. Westbrook, Maine USA.

 

Feline Haemotropic Mycoplasma (FHM) Tests and Profiles

Test CodeName and Contents

HMY

Feline Haemotropic Mycoplasma (FHM) RealPCR™ Test

Mycoplasma haemofelis (formerly Haemobartonella felis), Candidatus Mycoplasma haemominutum and Candidatus Mycoplasma turicensis RealPCR™ tests

 

Specimen requirements:

2 mL EDTA whole blood (LTT); keep refrigerated; collect sample prior to antibiotic administration.

  • When ordering an FHM profile that includes a CBC, chemistry or T4, also submit 2 mL serum, 1 mL EDTA whole blood (LTT) and 2 blood smears.
  • When ordering an FHM profile that includes a urinalysis, also submit 5 mL urine in a sterile container.

 

Feline Immunodeficiency Virus (FIV) Test

A highly specific diagnostic that detects the presence of FIV nucleic acid to confirm active infection

The IDEXX Feline Immunodeficiency Virus (FIV) RealPCR™ Test is the first highly specific diagnostic that detects the presence of FIV nucleic acid to confirm active infection. However, a negative FIV RealPCR Test result does not rule out infection. Rather, it can indicate one of three situations: the cat may not be infected; it may be infected, but nucleic acid is below limit of detection; or the cat may be infected with an uncommon strain not currently detected by PCR.

 

Additional Considerations for the FIV RealPCR Test

  • FIV serology testing, as with the SNAP® FIV/FeLV Combo Test and the SNAP® Feline Triple® Test, remains the first-line diagnostic because of its high sensitivity and specificity.
  • FIV RealPCR Test should only be considered in serologically positive cats when vaccination history is unknown or when cats have been vaccinated for FIV but infection is still suspected.
  • Although the FIV RealPCR Test is highly specific for infection, it cannot “rule out” infection or determine FIV vaccination status.
  • Given the limitations of interpretation, the FIV RealPCR Test should not be used to alter decisions regarding FIV vaccination protocol.
  • FIV PCR is not recommended as a screening for FIV.
  • Consider the SNAP Feline Triple IDEXX Advantage solution for your comprehensive diagnosis.

 

Feline Immunodeficiency Virus (FIV) Test

Test CodeName and Contents

FIR

FIV RealPCR™ Test

 

Specimen requirements:

2 mL EDTA whole blood (LTT); keep refrigerated.

 

Feline Leukaemia Virus (FeLV) Test

Consider the SNAP Feline Triple IDEXX Advantage solution for your comprehensive diagnosis.

 

Feline Leukaemia Virus (FeLV) Test

Test CodeName and Contents

FLP

FeLV RealPCR™ Test

 

Specimen requirements:

2 mL EDTA whole blood (LTT); keep refrigerated.

 

RealPCR™ Specimen Collection Guidelines

PCR testing offers a versatile testing modality for infectious diseases. However, accurate results depend on collecting the appropriate sample type for the clinical manifestation. Provided below are detailed protocols for each sample type.

All samples should be kept refrigerated, unless otherwise indicated. Samples should be collected prior to antibiotic or antifungal treatment directed against the organism of interest. Samples received within 72 hours of collection are preferred, but most samples are stable for up to 10 days if kept refrigerated, unless otherwise indicated.

Whole Blood: 2 mL EDTA whole blood (LTT).

Feces: 5 g fresh faeces (1 g minimum) in a sterile container (preferred) or empty, clean container.

Urine: 2 mL in a sterile container.

Fluid (CSF, pleural effusion, respiratory wash samples, uveal fluid, ascites, abscess aspirates, etc.): 0.5 mL (0.1 mL minimum) in a sterile tube (LTT preferred, plain RTT or plain plastic tube).

Tissue Aspirates: Submitting tissue aspirates on a swab (see below) improves stability of the sample. 1–3 unstained slides (keep at room temperature) are also acceptable specimens but may result in lower sensitivity.

Suggested Protocol:

  1. Aspirate tissue into the hub of the needle using standard techniques.
  2. Remove the syringe from the needle and fill syringe with air.
  3. Reattach the syringe to the needle, and squirt out aspirate onto the end of a dry, plastic-stemmed swab.
  4. Submit the swab in a RTT or empty, sterile tube.

Tissue Biopsies: PCR testing can be performed on previously submitted histopathology samples (formalin-fixed, paraffin-embedded tissue). Fresh, refrigerated tissue samples submitted within 24 hours of collection are also acceptable.

Swabs: Submit dry, plastic-stemmed swabs, without transport media, in a RTT or in an empty, sterile tube. Respiratory samples may include a deep pharyngeal swabs (with visible organic material on swab; please rub firmly), and a conjunctival swab (wipe eye clean; swab inside of eyelid), which can be submitted together in the same tube, or a nasal swab (equine respiratory panels or systemic mycoses). Collect samples prior to fluorescein staining. When acquiring a pharyngeal swab, precautions should be taken to prevent patient from biting and/or ingesting part of swab.

 

URD Samples: More than one way to swab a cat?

  

Submitting samples for the Feline URD Panel is easy. To optimize the diagnostic sensitivity of this panel, please submit samples from both sources as listed below.

 

Collection materials:

  • Two plastic-stemmed swabs.
  • A RTT or an empty, sterile tube.

 

Sample sources:

  • Deep pharyngeal swab—Rub firmly to ensure there is visible organic material on the swab.
  • Conjunctival swab—Wipe the eye clean and swab the inside of the eyelid.

 

Submitting samples:

  • The swabs should be placed together in the same RTT or in an empty, sterile tube. Do not put swabs into transport media.
  • Keep the tube with the swabs refrigerated until sending to the lab.
 

Frequently Asked Questions about IDEXX Real-Time PCR

 

What is PCR?

Polymerase chain reaction (PCR) is a simple yet elegant process that enables the production of virtually unlimited copies of genetic material in the laboratory.

 

How is real-time PCR different from conventional PCR?

Real-time PCR is a safer, faster and more robust variation of PCR. Conventional PCR harbors more risk for cross-contamination, is more time consuming and does not allow a quantitative assessment of the quality of the DNA extracted from the sample material.

 

Does your test differentiate between vaccinations and infection?

For most of the inactivated vaccines, real-time PCR does differentiate between vaccination and infection. Real-time PCR does not detect the presence of antibodies; therefore, a positive result indicates presence of the pathogen. For this reason, PCR has a higher association with disease than with antibody detection. Detection of viral DNA/RNA may occur within a few weeks after vaccination with a modified live vaccine (MLV) while the vaccine virus is replicating. We recommend a three-week interval after the last vaccination to prevent detection of any DNA originating from the vaccine.

 

What does a positive or a negative result mean?

A positive result confirms the presence of the pathogen. 

A negative result means the pathogen is either not present, below the limit of detection, not detectable by the current PCR assay or that the sample quality was low.

 

What is the limit of detection?

Detection limit of a method is defined in various ways. The most accepted definition is the target nucleic acid concentration or number of molecules that can still be amplified in 95% of the experiments.

 

What is the diagnostic sensitivity and specificity?

The diagnostic sensitivity describes the probability of a true positive result when the PCR is tested in a positive population. In other words, higher sensitivity means higher probability that you’ll correctly identify all positive samples and won’t miss a diagnosis. The diagnostic specificity is described as the probability of a true negative result when the PCR is tested in a negative population. Higher specificity is the probability that the test is really detecting what you’re testing for and not something unrelated. Therefore, higher specificity increases the probability that a positive result is due to the agent.

 

If a test comes back negative, but infection is still suspected, how long should I wait to retest?

This depends on the presence or absence of clinical signs, the pathogen(s) suspected and assay being used. In general, a two- to four-week waiting period is recommended.

 

Should I run another confirmatory test alongside the PCR test?

Yes. PCR is a complementary diagnostic procedure and should be run parallel with other diagnostic assays. Depending on the pathogen, this could include complete blood analysis, serology, antigen detection systems (such as SNAP® tests), etc.

 

How long can samples be stored in the refrigerator?

Samples can be stored in a refrigerator (4°C) for up to three days (over the weekend).

 

How should samples be stored?

Fluids (peritoneal/pleural fluid, whole blood, faeces) and swabs can be stored for three days (over the weekend) in a refrigerator (4°C).

Fresh tissues should be sent on ice within 24 hours. Tissues can be stored in a standard freezer at -20°C for up to one week. Paraffin-embedded tissues are acceptable.

 

How should samples be sent?

If you use a courier pickup, samples can be submitted with your other laboratory samples. All samples should be shipped according to applicable government  regulations. Send samples in an insulated package with ice packs. Samples should be placed in a secondary container within the package, in case of damage during shipping. Absorbent material should be included in the secondary container in case of leaks or breaks in the primary container.

 

What sample type should I submit?

The sample type is specific for each pathogen. Please refer to the specimen requirements for each test on the RealPCR Tests tab.

 

Test/PanelSpecimen Requirements

Feline Hemotropic Mycoplasma (FHM) RealPCR™ Test

Feline Immunodeficiency Virus (FIV) RealPCR™ Test

Feline Leukaemia Virus (FeLV) RealPCR™ Test

Whole blood (2 mL lavender-top tube, EDTA); keep refrigerated.

Feline Corona Virus (FCoV) RealPCR™ Test

0.3mL pleural or abdominal effusion (preferred specimen), CSF, EDTA whole blood, 10g faeces, fresh, frozen tissue (spleen, liver, kidney, lung), tissue embedded in parrafin, or tissue aspirates.

Submit fluids of faeces in plain (red topped tube) or sterile tube; keep refrigerated. 

Feline Upper Respiratory Disease (Feline URD) RealPCR™ Panel

Sample type: Deep pharyngeal swab (with visible organic material on swab; please rub firmly) and a conjunctival swab (wipe eye clean, swab inside of eyelid). Please submit dry, plastic-stemmed swabs, without transport media, in the same serum tube or an empty, sterile tube; keep refrigerated.

Canine Respiratory Disease (CRD) RealPCR™ Panel

Sample type: Deep pharyngeal swab (with visible organic material on swab; please rub firmly) and a conjunctival swab (wipe eye clean, swab inside of eyelid). Please submit dry, plastic-stemmed swabs, without transport media, in the same serum tube or an empty, sterile tube; keep refrigerated.

Canine Diarrhea RealPCR™ Panel

Feline Diarrhea RealPCR™ Panel

Sample type: 5 g of fresh fecal material; 1 g minimum in a sterile container; keep refrigerated.

Leptospira spp. RealPCR™ Test

Whole blood (2 mL lavender-top tube, EDTA) and 2mL urine in a sterile container; keep refrigerated

Tick/Vector Canine Comprehensive RealPCR™ Panel with Lyme Quant C6 Test

Whole blood (2 mL lavender-top tube, EDTA); keep refrigerated.

When ordering panels that include Borrelia spp. test, also submit 1mL serum.

 

How long are samples stored after they are processed?

Samples will be kept up to three days after receipt for reanalysis. The extracted DNA is stored for one year and can be used for reanalysis or further testing.

 

How do I confirm whether or not my sample has been received?

Check on the status of your sample online at www.vetconnect.com or call IDEXX at 1300 44 33 99. PCR testing is run daily at our Sacramento facility and you can expect results in 7–10 days.

 

Who do I contact with any questions or concerns?

For more information about IDEXX RealPCR™ panels and stand-alone tests, call our Internal medicine specialists at IDEXX Reference Laboratories at 1300 44 33 99, option 1, option 2. IDEXX’s board-certified internal medicine specialists are available to help answer your questions, such as:

  • When to use a PCR assay
  • How to interpret results
  • Treatment recommendations

 

What RealPCR™ tests do you offer?

Please consult the IDEXX Directory of Products & Services (DOPS) for a full listing of RealPCR™ tests.

 

Can additional testing be requested after a sample has already been processed?

The original samples are stored for three days. However, the extracted DNA from the sample is stored for one year. Additional tests can be requested on stored DNA when appropriate. For example, if originally only a distemper PCR was ordered, a full canine respiratory panel can be performed on the stored DNA if the original sample was a conjunctival swab and pharyngeal swab and not whole blood.

 

What is the turnaround time for samples submitted to your facility?

PCR tests for all pathogens are run daily Monday through Friday. Turnaround time for running a sample is 24 hours after receipt of the sample at the Brisbane facility. Samples delivered on Saturday or Sunday are processed on Monday. Including transport time, you can expect results in 1-3 working days.

 
 
PCR testing is a service performed pursuant to an agreement with Roche Molecular Systems, Inc.