IDEXX > Dairy Testing > Drug Residue Training > Troubleshooting
 

Troubleshooting


SNAP - No Color Development No Colour or Poor Colour Development
 
SNAP - High Background High Background Colour
 
SNAP - Poor Spot Quality Poor Spot Quality or Streaking
 
 
 
 
 
1. Introduction
2. Reasons for Contamination
3. Drug-Residue Avoidance
4. When and Why to Test
5. SNAP Technology
6. How to Use SNAP
7. Troubleshooting
8. Bulk-Tank Testing
 
Sign up for a FREE copy of the Antibiotic Drug-Residue Training CD (as seen here) and learn how testing with the rugged SNAP test is very simple and affordable.
 
 
No Colour or Poor Colour Development
  • Test may not have been prespotted
  • Pellet in tube may have been stuck at top and discarded inadvertently
  • Heater may not have been up to temperature, 45°C ±5°C
  • Sample may not have been incubated for the full 5-minute incubation time
  • SNAP® device may not have remained on heater for the 4-minute colour-development time
  • SNAP device may have been presnapped or not completely snapped
  • Rerun test

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High Background
  • Heater temperature may have been above 50°C
  • Sample may have been spilt onto results window when pouring it into sample well
  • SNAP device may have been activated (snapped) late
  • SNAP device may not have been fully activated (snapped)
  • Rerun test

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Poor Spot Quality or Streaking
  • Abnormal-looking milk may have been used, sample may be too thick to flow
  • SNAP device may have been activated too early or too late
  • Air temperature may be too hot or too cold, air temperature should be 18°–29°C
  • Heater temperature may have been outside of range, 45°C ±5°C
  • Pellet in sample tube may have been hydrated, often caused by taking test device out of foil prior to running
  • SNAP device may not have been activated (snapped) completely
  • Test device may have been removed from heater block prior to the full 4-minute colour-development time
  • Rerun test

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Discrepant Results—Visual vs. SNAPshot® Reader
  • May be spot quality issues on device (see above)
  • Background may not be clear, known as "high background" or "high blue" (see above)
  • Test device may have been removed from heater block prior to the full 4-minute colour-development time
  • Rerun test

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False-Positives
  • Heater temperature may have been above 50°C
  • Sample may have been over-incubated, correct incubation is 5 minutes
  • SNAP device may have been activated (snapped) too late
  • SNAP device may have been read before the full 4-minute colour-development time
  • Poor sample integrity, sample must be well-agitated
  • Rerun test

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False-Negatives
  • Heater temperature may have been below 40°C
  • Sample may have been under-incubated or incubation step skipped completely
  • Poor sample integrity, sample must be well-agitated
  • Rerun test

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