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VetStat® Analyser Principles of
Operation
The VetStat analyser is a microprocessor-based instrument that
measures optical fluorescence from discreet sensors called optical
electrodes (optodes). A disposable, single-use cassette contains all
of the elements needed for calibration, sample measurement and waste
containment. Specific calibration information from the cassette is
read into the analyser by swiping the cassette package through the
bar-code reader. The cassette is then placed into the measurement
chamber.
 The
analyser warms the cassette to 37.0° ±0.1°C (98.6°
±0.1°F), and performs a calibration verification on the
sensors for PCO2 and PO2 by
passing a precision calibration gas mixture across the optode sensors.
The pH and electrolyte channels are calibrated with precision buffer
solution contained in the cassette. When calibration is verified, the
analyser aspirates the blood sample into the cassette and across the
optode sensors. After equilibrating with the blood sample,
fluorescence emission is then measured.
After a single measurement, the cassette containing the blood
sample is removed from the analyser and discarded. The analyser
contains no reagents, blood or waste.
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